Multiplex Co-Amplification of 24 Retinoblastoma Gene Exons after Pre-Amplification by Long-Distance PCR
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چکیده
منابع مشابه
Multiplex co-amplification of 24 retinoblastoma gene exons after pre-amplification by long-distance PCR.
Polymerase chain reaction (PCR) amplification has become the method of choice for preparing the DNA template in mutation analysis from complex mixtures of DNA or RNA molecules. This strategy is optimal for small genes or genes with mutational hotspots. However, PCR-based mutation detection is neither labour nor cost effective when large or multiple genes, with many target fragments, are involve...
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A De Luca, I Bottillo, M C Dasdia, A Morella, V Lanari, L Bernardini, L Divona, S Giustini, L Sinibaldi, A Novelli, I Torrente, A Schirinzi, B Dallapiccola . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....
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We have found that assembling the reaction mixture at a temperature greater than the annealing temperature improved both product yield and specificity of PCR. When reactions were maintained at 70°C in a dry heating block during addition of denatured samples to aliquotted reagent master mix, a reproducible increase in product yield was observed compared to duplicates maintained at room temperatu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.3.538